This study was performed to develop an automated apparatus to study dyamic organ culture. In order to evaluate its efficiency, the viability of adult rat liver slices, cultured for 48 hours was confirmed morphologically and
biochemically.
The newly-developed culture apparatus consisted of four discrete components : the gas supplying system, the motor(rotational) system, the sterilizing system and the pressure controlling system.
The specimens were obtained from an adult rat liver sectioed into thickneses of 260 m and widths of 2mm2 and cultured for 48 hours in the morphological observation the criteria for the viable cells under the light microscope were
prominent nuclei and a deeply basophilic cytoplasm. Most of cell wre biable throughout the entire thickness f the slices, and no central necrosis was seen. Electronmicroscopy showed most of the cells retained their morphological
structures
for at least 48 hors, except for a slight loss of hetrochromatin.
The mean viabilities of the cultured slices and those of the cells in those slices were 7.17?5.0% and 76.0?11.4%, respectively.
Secondly, slice LDH leakage into the cultured medium and ATP-& DNA-contents of the cultured slices were measured for biochemical evaluation. The level of LDH activity increased in the specimens obtained between 5 minutes for
biochemical evaluation. The level of LDH activity increased in the specimens obtained between 5 minutes and 1 hour followed by no noticeable changes until 24 hours.At any sampling time significant changes were not shown in the
contents
of
ATP & DNA.
From the above results the newly-developed culture apparatus provided an effective method for the culture and biochemical maintenance of adult rat liver for 48 hours with minimal loss of biochemical function.
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